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dot blot-protoplasts


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#1 biology_06er

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Posted 10 January 2012 - 05:40 PM

A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution of it...so I was told do 4x 10 fold diltuin...does this mean if I started with 1:10 my next wld be 1:100, 1:1000, 1:10000/---is this right? Is there a “certain” starting dilution I should start with?

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#2 mdfenko

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Posted 11 January 2012 - 07:53 AM

your calculation results are correct for serial 10x dilutions.
talent does what it can
genius does what it must
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