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LENTIVIRUS transfection efficiency


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#1 gwenywar

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Posted 10 January 2012 - 07:01 AM

Hello,

does anyone have some numbers on transfection efficiency of lentivirus (or adenovirus) in hippocampal cell cultures?

I am infecting these cultures at the moment and get about 10% transfected cells but I would need 50% or more but I am not sure if that is realistic!

I hope someone can help, all publications just give the infection units which are not of much use to me at the moment!

Cheers!

#2 neuropath

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Posted 19 January 2012 - 06:26 PM

What species are the hippocampal cultures from and how did you prepare your virus?




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