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wet chamber for ISH

in situ wet chamber hybridization

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4 replies to this topic

#1 easy_o

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Posted 09 January 2012 - 08:27 AM

Hello everybody,
our lab performs in situ hybridizations on early mouse embryo (E.6.5-7.5) sections. Actually, we get quite nice results, however, our major problem is that sometimes our glass slides dry out when we incubate our probes over night in our self made wet chambers in the hybridization oven (68°C). If anyone has a simple and cheap solution we would be very grateful.

Best,
easy_o

#2 phage434

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Excellent

Posted 09 January 2012 - 09:54 AM

Plastic zip lock bag?

#3 easy_o

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Posted 09 January 2012 - 10:31 AM

I like your idea. I will give it a try and reply after my next experiment.
Thanks in advance.

#4 bob1

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Excellent

Posted 09 January 2012 - 04:22 PM

How about a Clik Clak box or perhaps using square petri dishes sealed with parafilm?

#5 Rsm

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Posted 10 January 2012 - 12:19 AM

Use PAP pen and cover with Parafilm...

My favourite is this one:
http://www.cosmobio....p?entry_id=3635
but not really cheap :(
I got soul, but I'm not a soldier





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