3 replies to this topic

[Biouday]

Hi,

Am looking for very specific marker for Vascular smooth muscle cell marker.


I used Anti-alpha smooth muscle Actin antibody do any one know other than this.

I will really appreciate your comments,
Thanks in advance

[bob1]

I don't think that alpha actin is specific to smooth muscle, just that it is more abundant in smooth muscle than in other cell types.

[Biouday]

bob1, on 09 January 2012 - 04:25 PM, said:

I don't think that alpha actin is specific to smooth muscle, just that it is more abundant in smooth muscle than in other cell types.

Yes last time i used enodthelial cells as negative control for immunostating , along with Smooth muscle cells, but i saw week and positive expression in endothelial cells.

ie what am looking for very specific marker for Smooth muscle cells.

And i checked all company website to know how they are charaterising the smooth muscle cells most of them are using the same Anti-alpha smooth muscle Actin antibody.

Edited by Biouday, 10 January 2012 - 03:00 AM.

[bob1]

It seems that there is no one marker for smooth muscle, you may have to use more than one.  One paper I found recommended SMα-actin, SMC myosin heavy chain (SM-MHC), Calponin, SM22α, and H-caldesmon.