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Vascular Smooth Muscle cell Marker


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#1 Biouday

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Posted 09 January 2012 - 05:07 AM

Hi,

Am looking for very specific marker for Vascular smooth muscle cell marker.


I used Anti-alpha smooth muscle Actin antibody do any one know other than this.

I will really appreciate your comments,
Thanks in advance

#2 bob1

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Posted 09 January 2012 - 04:25 PM

I don't think that alpha actin is specific to smooth muscle, just that it is more abundant in smooth muscle than in other cell types.

#3 Biouday

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Posted 10 January 2012 - 03:00 AM

I don't think that alpha actin is specific to smooth muscle, just that it is more abundant in smooth muscle than in other cell types.


Yes last time i used enodthelial cells as negative control for immunostating , along with Smooth muscle cells, but i saw week and positive expression in endothelial cells.

ie what am looking for very specific marker for Smooth muscle cells.

And i checked all company website to know how they are charaterising the smooth muscle cells most of them are using the same Anti-alpha smooth muscle Actin antibody.

Edited by Biouday, 10 January 2012 - 03:00 AM.


#4 bob1

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Posted 10 January 2012 - 12:37 PM

It seems that there is no one marker for smooth muscle, you may have to use more than one. One paper I found recommended SMα-actin, SMC myosin heavy chain (SM-MHC), Calponin, SM22α, and H-caldesmon.




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