glucose concentration, pyruvate and phenol red in DMEM
Posted 08 January 2012 - 07:07 PM
I would like to know what is the appropriate concentration of Glucose in DMEM medium for ordinary maintenance of human cancer cell lines, is it 1 g/L(5.5mM) or 4.5 g/L (25mM). Also should it contain pyruvate or not? should it contain phenol red or not?
Posted 09 January 2012 - 04:27 PM
Posted 10 January 2012 - 01:03 AM
Despite my lack of experience,
I read that different medium composition can cause ur adherent cells not to attach for example.
Posted 11 January 2012 - 07:08 PM
comments are appreciated
Posted 12 January 2012 - 11:47 AM
Regards phenol red: Some cell lines express oestrogen receptors, which as phenol red is an oestrogen analogue (albeit weak), causes internalization of the receptor and a corresponding decrease in the growth rate of the cells, in theory at least.
Posted 15 January 2012 - 05:00 PM
Edited by yobou, 15 January 2012 - 05:50 PM.