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Trouble with the GFP signal

Started by athena3764, Jan 06 2012 03:29 PM

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#1 athena3764

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Posted 06 January 2012 - 03:29 PM

I'm generating a cell line stably expressing a GFP fusion protein.  I have one clone that is fluorescent under the microscope. However, I cannot detect the GFP fusion signal by western blot with anti-GFP antibody =(  The antibody is fine as indicated by the positive control.   Is fluorescence microscopy is more sensitive than western blot?

Thanks!
Athena

#2 bob1

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Posted 06 January 2012 - 04:09 PM

possibly, or it could be tha you are seeing auto-fluorescence of the cells rather than GFP.
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