Difference between EST and cDNA?
Posted 06 January 2012 - 09:13 AM
I am not in molecular biology field, but I need to understand more about EST(expressed sequence tags). I am wondering how is it generated? Is it from mRNA? After reading info in Wiki, I think cDNA is generated from mRNA as well. So what is the difference of the two? I am really confused about these two. It seems that some mature peptides are in cDNA but not in EST yet. Any explanations? Thank you very much.
Posted 06 January 2012 - 04:38 PM
ESTs are generated by sequencing cDNA, which itself is synthesized from the mRNA molecules in a cell
So what is the difference of the two?
EST's are small sequences generated from sequencing cDNA, but only a small portion, this can be used as a sign to identify the gene and does not reveal the whole gene sequence as cDNA does
Edited by Felipillo, 06 January 2012 - 04:39 PM.
Posted 06 January 2012 - 05:14 PM
Posted 06 January 2012 - 08:30 PM
Posted 07 January 2012 - 10:30 PM