Posted 09 April 2003 - 12:59 PM
Does the commercial PCR products such as the ones from Sigma aldrich , not have the 5' phosphate ends? Its a major problem since i am going for blunt end ligation of PCR product directly and I will ve to know if i ve to do CIPPing of the vector.
Posted 20 April 2003 - 05:49 AM
I have myself found the answer for this
The commercially synthesized primers come without a 5'phosphate ends(primers from organisations such as Sigma aldrich) and under special circumstances they also provide primers with 5' phosphate group at an extra cost.
Hope this will be useful for others
Posted 24 April 2003 - 08:08 AM