In many protocols involving some form of treatment on cells, for example immunostaining protocols, I run into many 'recommended' solutions that the protocols suggest me to use on the cells for certain steps such as a pre-extraction step for immunostaining MDCK's tight junctions.
* Pre-extract with 0.2% Triton X-100 in 100mM KCL, 3mM MgCl2, 1 mM CaCl2, 200mM sucrose, and 10mM Hepes (pH 7.1) for 2 min on ice, making sure that buffer is gently applied to cells.
I know this step is definitely crucial but for curiousity's sakes as well as for me to simply learn more about how certain solutions with certain ions and their concentrations affect - structure + physiology, are there any certain websites or books where I can learn more about how these solutions affect cells?
I am very weak in cell biology, and any recommendations that may help me learn more about cells is greatly appreciated.
Thanks for the time!
Edited by Taminem2012, 04 January 2012 - 10:47 AM.