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Plasmid sequencing


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#1 EgyBola

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Posted 04 January 2012 - 07:28 AM

I did transformation of a pMIR-REPORT plasmid and when I sequence, I get high background in the sequence. However, there's always 90%+ similarity when I align the sequences. The primers are the ones suggested by the plasmid company and were tested before.

Can anybody help where this comes from?

Thanks in advance.

Cheers,
Bola

#2 phage434

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Posted 04 January 2012 - 07:10 PM

This sounds like a sequencing problem. Perhaps you are using either too much or too little DNA in the sequencing reaction. Can we see an .abi file?




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