In qRTPCR using taqman, do we need to design both primers and probe. I ill be using Taqman expression assay mix. Please help as Iam totally new to this taqman thing..
0
4 replies to this topic
#2
-
- Active Members
-
- 577 posts
Brain on a stick
Posted 05 January 2012 - 02:02 PM
Try to find something about primer and probe design and then use design software like Primer Express (you should have it if you have ABI machine) , or free aplication like primer3.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
#3
-
- Active Members
-
- 11 posts
member
Posted 06 January 2012 - 08:38 AM
Thanks for replyin bt in taqman qrtPCR do we hav to design for both primer pairs n probe? I thought we just hav to design for probe...
#4
-
- Active Members
-
- 577 posts
Brain on a stick
Posted 06 January 2012 - 12:54 PM
Sure, you have to design both, for a probe to anneal, your specific region must be amplified with primers.
And please use all the letters, it's really difficult to read.
And please use all the letters, it's really difficult to read.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
