4 replies to this topic

[DDLJ]

In qRTPCR using taqman, do we need to design both primers and probe. I ill be using Taqman expression assay mix. Please help as Iam totally new to this taqman thing..

[Trof]

Try to find something about primer and probe design and then use design software like Primer Express (you should have it if you have ABI machine) , or free aplication like primer3.

[DDLJ]

Thanks for replyin bt in taqman qrtPCR do we hav to design for both primer pairs n probe? I thought we just hav to design for probe...

[Trof]

Sure, you have to design both, for a probe to anneal, your specific region must be amplified with primers.
And please use all the letters, it's really difficult to read.

[DDLJ]

thanks. ok i will...