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qRTPCR using taqman


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#1 DDLJ

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Posted 04 January 2012 - 06:58 AM

In qRTPCR using taqman, do we need to design both primers and probe. I ill be using Taqman expression assay mix. Please help as Iam totally new to this taqman thing..

#2 Trof

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Posted 05 January 2012 - 02:02 PM

Try to find something about primer and probe design and then use design software like Primer Express (you should have it if you have ABI machine) , or free aplication like primer3.

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#3 DDLJ

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Posted 06 January 2012 - 08:38 AM

Thanks for replyin bt in taqman qrtPCR do we hav to design for both primer pairs n probe? I thought we just hav to design for probe...

#4 Trof

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Posted 06 January 2012 - 12:54 PM

Sure, you have to design both, for a probe to anneal, your specific region must be amplified with primers.
And please use all the letters, it's really difficult to read.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#5 DDLJ

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Posted 09 January 2012 - 12:14 PM

thanks. ok i will...




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