Posted 03 January 2012 - 10:46 AM
Posted 05 January 2012 - 12:49 PM
Probably you were told to use SURE cells because this plasmid forms a secondary structure that normal Eco strains may cut out. If you don't have SURE cells, can't get ones and have enough of plasmid, you could try DH5aplha, but you may have serious problems with propagation in this strain so better check the plasmid preps, if they contain the complete plasmid. Maybe you'll find you can't grow it there.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon