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Plasmid isolation Qiagen

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#1 Karenarabella



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Posted 03 January 2012 - 08:35 AM

Plasmid isolation - hi all. Anyone ever tried isolating a plasmid using a Qiagen gel extraction kit? I have all mini prep buffers from qiagen kit but am out of columns. I don't really have time to wait for a new kit to arrive and I'm considering just using a gel extraction column. Anyone ever tried that before?


#2 hobglobin


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Posted 03 January 2012 - 12:04 PM

You also can regenerate the old ones, if you kept them. There are commercial solutions for this (eg from this company) and also hand-made protocols with self-made solutions. I don't remember the protocols, but a search here or with google should give some results.

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#3 phage434



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Posted 03 January 2012 - 06:15 PM

The columns are the same in both kits, except (possibly) for the color.

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