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his tagged protein


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#1 claire84

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Posted 03 January 2012 - 12:58 AM

Hi everybody,
I have a question for you..
I have to purchase HIV-1 aspartic proteinase to perform an inhibition assay and Sigma aldrich has only the his-tagged version. Can I buy it?Is it suitable for my test?His-tag are used in the purification process or in affinity test, is it right?Can the presence of it influence my inhibition assay?

Thank you

#2 Abdulrahim Alawneh

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Posted 05 January 2012 - 09:35 PM

i guess u need an active proteinase
and usually his tag does not interfere with activity, also, u can check product activity on their website, if its active then u dont need to worry i guess




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