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how to explain this arm-pcr result


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4 replies to this topic

#1 NHANG MUOI

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Posted 01 January 2012 - 08:14 PM

we are first year student.
hope your help!
thank you so much!

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#2 YanYan

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Posted 02 January 2012 - 06:44 AM

help us please . We dont know why 2 times of running ARM-PCR so different :(Posted Image

#3 NHANG MUOI

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Posted 03 January 2012 - 01:40 AM

i answered that because off contaminant, but our instructor didnt agree and want us give more details, more other options.

#4 Sunflower_gl

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Posted 04 January 2012 - 02:43 AM

Do you have a control negative (H2O)? If the band also appear with the control negative, it means your preparation is contaminated by gDNA I think. Let see in the topic " PCR Contamination issues" and verify step by step.
Good luck


#5 YanYan

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Posted 07 January 2012 - 02:11 AM

we didnt make negative control :( Thats why we are not sure it has contamination or not ! In the slide of ARM-PCR , we confused why do we have a light bright band underneath the 100bp band in the ladder well dear
Hope to hear from u soon
thanks




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