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Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati

Riboprobe Fluorescence in sit

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#1 MishMish

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Posted 01 January 2012 - 02:45 PM

I am a little stuck on whether my ribo-probe has worked, I have used a dig-labelled probe with TSA amplification and previously it worked. However this time I used a probe to label PRDM-16 mRNA and got what seemed to be nuclear staining (as it co-stains with DAPI in brown fat). I know that as a protein it is expressed in the nucleus, but surely as mRNA it should be transported to the cytoplasm.... and so stain there? I haven't made a sense control probe to compare it to as I ran out of time... Any idea

Thanks

Edited by MishMish, 01 January 2012 - 05:04 PM.


#2 bob1

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Posted 02 January 2012 - 06:35 PM

You need the controls to be sure...




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