Posted 05 January 2012 - 01:03 PM
You need standards of known copy number/concentration to do absolute quantification. You need calibrator and reference gene to do relative quantification. If you have both on a plate/s when you run it you can analyse both ways. Otherwise not. The machine measures fluorescence and Cts, analysation software modules are usually independent on the setting but you need to have WHAT to analyse.
I hope you just designed relative quantification experiment and included dilution series which were then mistaken as standards, because otherwise I honestly can't imagine how you could have RQ experiment, analysed as absolute. What exactly did you have on your assay?
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.