Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

qPCR statistics (ANOVA) after normalization.


  • Please log in to reply
No replies to this topic

#1 cells

cells

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 32 posts
0
Neutral

Posted 28 December 2011 - 10:27 PM

Hi,

I was wondering about performing statistical analysis of qPCR data after you have normalized all your data to your reference genes and you only have the fold changes (along with SEM) to work with. I was wondering if it's ok to use normalized data to do things like ANOVA. So the type of data I have is something like this:


---------------------------------------------------------------
| 24h | 48h | 72h |
---------------------------------------------------------------
DMSO | 1 +/- 0.2 | 1 +/- 0.15 | 1 +/- 0.12 |
---------------------------------------------------------------
100nM | 1.5 +/- 0.12 | 1.8 +/- 0.2 | 1.25 +/- 0.22 |
---------------------------------------------------------------
1uM | 2 +/- 0.21 | 2.5 +/- 0.25 | 1.78 +/- 0.23 |
---------------------------------------------------------------

Where x +/- y represents fold change +/- SEM...it's all relative to DMSO control so that ends up being 1 fold change. I was wondering if it's ok to do ANOVA analysis on this type of data or do you need to go back to your Ct values to perform ANOVA. Any help would be great as I'm kind of desperate. Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.