I'm a research intern at MD Anderson Cancer Center looking for tips to make my blots look "right." My problem that I've been facing is that I keep getting these weird lines. I defrost and use WCL with inhibitors on application. I will also add that I accidentally boiled these samples longer than usual (99C for 20 minutes). I usually do 10 minutes.
Edited by doctorlambchops, 28 December 2011 - 09:28 AM.