Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Pseudomonas aeruginosa growth very slow in N-minimal media

Pseudomonas N-minimal media growth

  • Please log in to reply
2 replies to this topic

#1 rameshcdri

rameshcdri

    member

  • Active Members
  • Pip
  • 14 posts
1
Neutral

Posted 27 December 2011 - 08:10 PM

Hi All,

I am growing Pseudomonas aeruginosa WT strain in N-Minimal media at low Mg++ concentration (8uM/ml). But, it is growing very slowly. I had grown PA in 25ml media from picking single colony, over night. But, I got only 2mg bacteria after lyophilization. On the basis of this observation I have two question.

1) Is that Okay, if i grow it for a longer time (more than 24hr)?
2) Will the bacteria begin to die, If it continues to grow for a longer time (24hr) due to Mg++ deficiency?

Kindly give me your suggestions.


Ramesh

#2 lamaksha77

lamaksha77

    member

  • Active Members
  • Pip
  • 16 posts
1
Neutral

Posted 30 December 2011 - 09:02 AM

If you are concerned over bacterial viability, measure cfu counts (by plating) and the OD600 at different stages of growth. If the OD600 stagnates, or the cfu counts drop, you have grown too far into the stationary phase. Personally, I think it will take longer than 24hours for the bacteria to reach stationary phase in minimal media (probably will take several days).

One tip is to first have a dense culture growing (stationary phase, probably growing for >24hours), and then sub-culture 1/100 or 1/50. This way your culture would reach exponential growth phase faster than if you started a culture by colony picking...

Cheers,

#3 rameshcdri

rameshcdri

    member

  • Active Members
  • Pip
  • 14 posts
1
Neutral

Posted 03 January 2012 - 07:19 AM

Thanks.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.