Help with some problems with Culturing Epithelial Cells
Posted 25 December 2011 - 05:30 PM
bobby has been culturing epithelial cells for experiments that he is performing in the laboratory. He has subcultured the cells many times, and has always fed the cells the proper medium. He recently ran an experiment and found that his cells are not growing the way that he previously observed. What are some reasons why these cells may not be acting as they did previously?
Since this was a free online lecture, the answer was not provided and since i am having the same issue in my lab, I am wondering what is the cause for this phenomenon and if you guys may know the answer. Also, if possible, where would I be able to read more about this?
Posted 26 December 2011 - 10:25 PM
- Mycoplasma contamination
- Prolong trypsinization
- Altered culturing conditions
- Improper cell-freezing
and many others
Posted 04 January 2012 - 10:39 AM
Posted 07 January 2012 - 09:02 AM
Posted 15 January 2012 - 01:33 AM
I don't know exactly the reasons but genetic instability or something else could be one possible explanation.