SDS-Electrophoresis, Dye color change
Posted 24 December 2011 - 07:22 AM
Ran a gel, gel ran fine, in fact, perfect, until about 1-2 cm from the bottom of the gel, the dye turned yellow, and wouldn't move any further. I assume the sample buffer (BMB) turned yellow due to acidity, but I'm at a loss to figure out exactly what is doing that, and how to stop it?
Gel still transfers just fine, and my proteins wavy up the top anyway (like 250 KD). But it's just a random and bugging me. Anyone come across this before?
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Posted 26 December 2011 - 02:05 PM
Edited by mdfenko, 26 December 2011 - 02:06 PM.
genius does what it must
i do what i get paid to do