Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

mir30-based shrna cloning-mismatch


  • Please log in to reply
1 reply to this topic

#1 Ambinlab

Ambinlab

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 65 posts
1
Neutral

Posted 23 December 2011 - 10:16 AM

Dear All,

I cloned a mir-30 based shrna on a lentiviral vector recently. I submitted for sequencing and found out that there is some mismatches on sense and antisense sequences but not mir30 context and loop sequence.

Any ideas why it is like that and how to avoid it?

Thanks

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 15 January 2012 - 12:13 PM

mismatches on sense and antisense sequences but not mir30 context and loop sequence.

It is not clear to me what you mean by "not mir30 context and loop sequence". Whether it is intended for a few mismatches between the sense and antisense sequences in the shRNA?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.