Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

stripping microarrays

Agilent microarray stripping RNase H

  • Please log in to reply
No replies to this topic

#1 coral_tech



  • Members
  • Pip
  • 4 posts

Posted 21 December 2011 - 12:17 PM


Has anyone had any luck stripping Agilent microarrays? The company doesn't want you to, but there are some published studies indicating there several methods for successful re-use (e.g. Zhang et al 2009, Hu et al 2005, Wu et al 2008, Hahnke et al 2007) . At >$1,000 per chip, getting more than one use would be appreciated.

Does anyone have any experience with E. coli RNase H for stripping? What is the temperature range? Optimal temperature is 37degC, but I'm interested in the upper temp limit without inhibiting activity too much (~90%).


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.