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stripping microarrays

Agilent microarray stripping RNase H

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#1 coral_tech

coral_tech

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Posted 21 December 2011 - 12:17 PM

Hi,

Has anyone had any luck stripping Agilent microarrays? The company doesn't want you to, but there are some published studies indicating there several methods for successful re-use (e.g. Zhang et al 2009, Hu et al 2005, Wu et al 2008, Hahnke et al 2007) . At >$1,000 per chip, getting more than one use would be appreciated.

Does anyone have any experience with E. coli RNase H for stripping? What is the temperature range? Optimal temperature is 37degC, but I'm interested in the upper temp limit without inhibiting activity too much (~90%).

Thanks





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