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HaCaT reference gene

HaCaT reference gene

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#1 EvilTwin

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Posted 19 December 2011 - 04:04 AM

I would like to know what reference genes you recommand for HaCaT cells. I've read that most of the so-called "housekeeping genes" are not stable in HaCaT and primary keratinocytes ( http://www.jdsjourna...0344-1/abstract ), while one group, on whose article I've based my experiments, used beta-actin which is described as one of the least appropriate reference genes. In my qRT-PCR i've used beta-actin and B2M as reference genes and none of them seems sufficiently stable.
Which genes do you use? Any ideas about how I can improve my experiments?

#2 Rsm

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Posted 19 December 2011 - 04:11 AM

You may want to use GeNorm and find the most stable genes yourself...
http://medgen.ugent....vdesomp/genorm/
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