How is it possible to check whether any published miRNA is located in introns of a certain gene(eg. TGF ?
checking whther there is any miRNA in introns of a specific gene.
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Posted 20 December 2011 - 06:18 AM
go to mirbase website. Download the gff file ( coordinates of each pre-miRNA). Check if they are in intron by overlapping with the intron coordinates. You can have the coordinates of exons (so introns too) in ensembl (biomart). Enjoy