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checking whther there is any miRNA in introns of a specific gene.


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#1 ali.protocol

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Posted 19 December 2011 - 03:22 AM

Hi,

How is it possible to check whether any published miRNA is located in introns of a certain gene(eg. TGF B)?

#2 NicoBxl

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Posted 20 December 2011 - 06:18 AM

go to mirbase website. Download the gff file ( coordinates of each pre-miRNA). Check if they are in intron by overlapping with the intron coordinates. You can have the coordinates of exons (so introns too) in ensembl (biomart). Enjoy




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