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HCL instead of imidazole!


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#1 biology_06er

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Posted 15 December 2011 - 08:50 PM

Hi there,

Soo today I eluted with MCAC-100 (or so I thought)..I realsied once i added reducing buffer i added 1M HCL (i have them in the same size bottle next to each other on my shelf)...so what i did was i just washed my column with MCAC-0 a few times and eluted with the correct imidazole concentration ...just wondering will this work..im running as gel as i write this but just wondering if the beads are still useable

thx
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#2 biology_06er

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Posted 15 December 2011 - 10:28 PM

or more likely will the HCl remains on the beads affect my protein :(

#3 mdfenko

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Posted 16 December 2011 - 07:19 AM

you may have hydrolyzed your protein. if the exposure was short then you may still have some useful protein. your gel will tell.
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