HCL instead of imidazole!
Posted 15 December 2011 - 08:50 PM
Soo today I eluted with MCAC-100 (or so I thought)..I realsied once i added reducing buffer i added 1M HCL (i have them in the same size bottle next to each other on my shelf)...so what i did was i just washed my column with MCAC-0 a few times and eluted with the correct imidazole concentration ...just wondering will this work..im running as gel as i write this but just wondering if the beads are still useable
Posted 15 December 2011 - 10:28 PM
Posted 16 December 2011 - 07:19 AM
genius does what it must
i do what i get paid to do