Hi there,
Soo today I eluted with MCAC-100 (or so I thought)..I realsied once i added reducing buffer i added 1M HCL (i have them in the same size bottle next to each other on my shelf)...so what i did was i just washed my column with MCAC-0 a few times and eluted with the correct imidazole concentration ...just wondering will this work..im running as gel as i write this but just wondering if the beads are still useable
thx
B_06er
0
2 replies to this topic
#2
biology_06er
-
- Active Members
-
- 53 posts
Enthusiast
0
Neutral
Neutral
Posted 15 December 2011 - 10:28 PM
or more likely will the HCl remains on the beads affect my protein 
#3
mdfenko
-
- Active Members
-
- 2,240 posts
an elder
77
Excellent
Excellent
Posted 16 December 2011 - 07:19 AM
you may have hydrolyzed your protein. if the exposure was short then you may still have some useful protein. your gel will tell.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
