I'm having some troubles to maintain my M-NFS 60, I tried a lot of things but this black cells are killing all the healthy cells that I have.
I would describe what is happening
The cells are ok, I have check everything (media, supplements, environment, and also my plasticware ) .
1 I thaw my vial and The first day cells appear to be ok-
2 The second day the cells divide but ... they start to die,
3 The third day the percent of dead cells is higher than viable cells
4 The fourth day all the cells are dead or apoptotic.
This black cell is acting like a Natural killer, and is killing and eating all my cells, also is to big to be a bacteria don't you think or a yeast?
Anybody has experienced this before ?
I would appreciate some help with this.