Posted 15 December 2011 - 11:41 AM
Let's say I am doing some work in a biosafety cabinet (which I will affectionately refer to as "the hood"). I've sterilized it, I've sterilized all my equipment, I've sterilized me, everything is sterile. I open a box of autoclaved pipette tips inside the hood.
I use a few, maintaining good laboratory practices while doing so. My experiment remains uncontaminated.
I close the autoclaved pipette tips inside the hood.
If I remove these tips from the hood, never opening them outside the hood at all, would the tips still be considered sterile? I.e., could I clean the outside of the box, replace it in the hood, open it and carry on as normal, or would they have to be reautoclaved before going back in the hood?
I hope this isn't a dumb question.
Posted 15 December 2011 - 12:01 PM
Posted 15 December 2011 - 12:26 PM
That is how work is normally done in biosafety hoods. For optimal performance, they should have as little stuff in them as possible, and be emptied before cleaning down after you have finished working.
I'm a new graduate student and my lab had been leaving the boxes stacked neatly in the hood. It worked well for a while (we have a very large hood, so they took up little space) but people started leaving other crap in there as well so everything's been stripped out. I've got my own stash of pipette boxes now but just wanted to check and make sure I wasn't going to contaminate anything.
Posted 15 December 2011 - 01:44 PM
As long as you keep it sealed, it should be fine to reuse those tips.
Posted 16 December 2011 - 06:13 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 05 January 2012 - 08:14 AM