No replies to this topic

[RagnhildK]

Dear All.

So I saw that "Carolin01" and "peanutnation" were discussing transfection efficiency in SH-sy5y.
Ive transfected my SH-sy5y with LPS 2000 serum free and got a renilla act. about 20, which is not alot over background but high enough to see differences in Luciferase activity.

I was thinking about transfecting with an eletroporator, does anyone a any experience doing that in SH-sy5y.
Also I have tried to use Lipofectamine LTX and FuGene which didnt work for me.

All comments are appreciated.


THANK you!!!