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Different transfection methods in SH-sy5y cells.

Started by RagnhildK, Dec 14 2011 01:44 PM

Sh-Sy5y transfection

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#1 RagnhildK

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Posted 14 December 2011 - 01:44 PM

Dear All.

So I saw that "Carolin01" and "peanutnation" were discussing transfection efficiency in SH-sy5y.
Ive transfected my SH-sy5y with LPS 2000 serum free and got a renilla act. about 20, which is not alot over background but high enough to see differences in Luciferase activity.

I was thinking about transfecting with an eletroporator, does anyone a any experience doing that in SH-sy5y.
Also I have tried to use Lipofectamine LTX and FuGene which didnt work for me.

All comments are appreciated.

THANK you!!!

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