Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Different transfection methods in SH-sy5y cells.

Sh-Sy5y transfection

  • Please log in to reply
No replies to this topic

#1 RagnhildK

RagnhildK

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 14 December 2011 - 01:44 PM

Dear All.

So I saw that "Carolin01" and "peanutnation" were discussing transfection efficiency in SH-sy5y.
Ive transfected my SH-sy5y with LPS 2000 serum free and got a renilla act. about 20, which is not alot over background but high enough to see differences in Luciferase activity.

I was thinking about transfecting with an eletroporator, does anyone a any experience doing that in SH-sy5y.
Also I have tried to use Lipofectamine LTX and FuGene which didnt work for me.

All comments are appreciated.


THANK you!!!





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.