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Transfection - Changing Media


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#1 vwracer06

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Posted 30 March 2003 - 10:36 PM

I am at the beginning stages of transfection and have added my transfection reagent and dna to my cells. My question is, now that I want to do my selection with g418, how often do I have to change my media? My media that i use contains 250ug/ml of g418. Once a week? Every other day?

Thanks

#2 navadann

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Posted 28 April 2003 - 05:41 AM

generally when i perform a transfection (g418 selection) i plate the cells with media which doesnt contain g418. This is so the cells can propagate. generally i will do this for 24-48 hours -depends on how the cells look-. i then add media with the selection drug -g418- and immediately i begin to see cell death. i change media every other day until clone screening is complete.




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