BSA concentration for blocking
Posted 14 December 2011 - 04:16 AM
1%? 5%? 10%?
Posted 14 December 2011 - 06:58 AM
I always had an alternate hypothesis....
Posted 14 December 2011 - 10:12 PM
Posted 14 December 2011 - 10:57 PM
Posted 15 December 2011 - 03:44 AM
I myself have generally had bad results with BSA blocking (high background). We use milk instead.
I found the opposite when doing a particular antibody. BSA blot was sparkling clean whilst milk gave alot of grey spots, smears and background after adding ECL. I think it has something to do with the primary antibody. I was detecting a phospho-protein. Milk was fine with the native form.
BSA is quite expensive so I was wondering if 1% does the job, it'll be good to save up on BSA stocks.
Posted 15 December 2011 - 12:05 PM