eIF4G western blot
Posted 13 December 2011 - 01:15 PM
I am having problems running eIF4G. Where I normally have a perfect line, I am seeing a bleached out area with a "blob" looking line underneath my target area. I have tried a different antibody from another company and still get the same results. This is happening to all my samples for this specific initiation factor. Any suggestions would be helpful. I have attached a picture of what I normally get and what my blots look like now. Thanks!
Posted 14 December 2011 - 12:53 AM
Posted 14 December 2011 - 12:49 PM
genius does what it must
i do what i get paid to do
Posted 14 December 2011 - 02:57 PM