2 replies to this topic

[drelamuruganvet]

I am using the following RBC lysis buffer composition for lysis of chicken RBCs

Ammonium chloride   8.3 g
Potassium bicobonate 1.0 g
EDTA 0.037 g

pH adjusted to 7.4 using 1N HCl

final volume  to 1 litre using distilled water

autoclaved and storage at room temperature

most references suggesting the above one but still  I am unable to lyse RBC completely

if anyone knows pl kindly reply

Edited by drelamuruganvet, 13 December 2011 - 07:03 AM.

[mdfenko]

don't autoclave bicarbonates. prepare them fresh and filter sterilize if necessary.

[drelamuruganvet]

mdfenko, on 13 December 2011 - 09:44 AM, said:

don't autoclave bicarbonates. prepare them fresh and filter sterilize if necessary.

thank you sir...