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RBC LYSIS BUFFER


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#1 drelamuruganvet

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Posted 13 December 2011 - 07:01 AM

I am using the following RBC lysis buffer composition for lysis of chicken RBCs

Ammonium chloride 8.3 g
Potassium bicobonate 1.0 g
EDTA 0.037 g

pH adjusted to 7.4 using 1N HCl

final volume to 1 litre using distilled water

autoclaved and storage at room temperature

most references suggesting the above one but still I am unable to lyse RBC completely

if anyone knows pl kindly reply

Edited by drelamuruganvet, 13 December 2011 - 07:03 AM.


#2 mdfenko

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Posted 13 December 2011 - 09:44 AM

don't autoclave bicarbonates. prepare them fresh and filter sterilize if necessary.
talent does what it can
genius does what it must
i do what i get paid to do

#3 drelamuruganvet

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Posted 13 December 2011 - 07:16 PM

don't autoclave bicarbonates. prepare them fresh and filter sterilize if necessary.




thank you sir...




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