Freezing of Protozoa (Toxoplasma)protozoa
Posted 13 December 2011 - 05:56 AM
Freshly thawed parasites have very low viability (10%~), I realized gradual freezing is important.
But my lab does not have the machine (for gradual freezing -1oC per minute). Is there other way to achieve gradual freezing ?
Thanks in advance for the help.
Please advise with your professional opinion.
Posted 13 December 2011 - 06:31 AM
This slow freezing is not as effective than controlled gradual freezing, but the cells should survive better this way than putting them straight to -80°C.....
Posted 13 December 2011 - 08:43 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 15 December 2011 - 10:32 PM
Posted 15 December 2011 - 11:01 PM
Posted 15 December 2011 - 11:11 PM
Posted 22 July 2012 - 05:46 PM
Posted 31 December 2012 - 05:45 AM
I just thaw normally like how to thaw cells.. but u have to infect the toxo immeadiately into mice intraperitoneally.. cos for me.. reinfection in cells doesnt work at all.. hope it works!!