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cytokine levels in brain tissue

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#1 jasmina



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Posted 13 December 2011 - 02:37 AM

I want to qantify one cytokine level in striatum protein extract with elisa and western blot.
I dont have signal in western blot.
I got weak signal in elisa in 150ug of protein extract, but no signal in lower contentrations of extracts.
I want to ask if there is any eventual way to improve my sandwich elisa, a part from using high protein extracts to optimize the conditions of elisa.
If there is another tool more efficient and easy to qantifiy my cytokine levels.
thank you for help

#2 PAO_ahac



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Posted 13 December 2011 - 06:57 AM

Improving your elisa:
Longer incubation time
Inclusion of PEG to increase binding
Sealing plate and shaking/mixing during incubation
Increasing incubation temperature
optimizing reagent volumes
More sensitive assay method

If available using pre-existing manufactured kit (providing analytical range is acceptable)

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