Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cytokine levels in brain tissue


  • Please log in to reply
1 reply to this topic

#1 jasmina

jasmina

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 134 posts
3
Neutral

Posted 13 December 2011 - 02:37 AM

I want to qantify one cytokine level in striatum protein extract with elisa and western blot.
I dont have signal in western blot.
I got weak signal in elisa in 150ug of protein extract, but no signal in lower contentrations of extracts.
I want to ask if there is any eventual way to improve my sandwich elisa, a part from using high protein extracts to optimize the conditions of elisa.
If there is another tool more efficient and easy to qantifiy my cytokine levels.
thank you for help

#2 PAO_ahac

PAO_ahac

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 94 posts
0
Neutral

Posted 13 December 2011 - 06:57 AM

Improving your elisa:
Longer incubation time
Inclusion of PEG to increase binding
Sealing plate and shaking/mixing during incubation
Increasing incubation temperature
optimizing reagent volumes
More sensitive assay method

If available using pre-existing manufactured kit (providing analytical range is acceptable)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.