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Luciferase assay (problem with pRL-tk)

luciferase

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#1 teageek

teageek

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Posted 12 December 2011 - 02:27 PM

I am trying to test the promoter that I am interested in.
I trransfected with pRL-tk for normalization.
For some reason, my luciferase did not changed much, instead, ranella luciferase changed 10 times (100,000).
In this case, I cannot calculated as 10 fold decrease, because RL is increased.
Anyway, has anyone have experience that RL-tk is regulated this much?
I can understand like 2 fold, which is changed by experimental conditions, but never like this.
Please help.





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