1 reply to this topic

[sikander]

Hi all,

I am studying the ERK activation in HT22 cells and for this first i probe my blot with total ERK and got good  signal and later on when i do stripping. After stripping the blot i prob the blot with phosphoERK antibody.

My total ERK gives very good signal, while there comes huge background in the phospho ERK probing.

Can anyone gives some suggestion.

thanks

[Biouday]

Hi

Did you check blots only with phospho ERK, is that good without background.
And my suggestion is always do PhoshoERK ( any phospho protein) before doing the total protein.