I am studying the ERK activation in HT22 cells and for this first i probe my blot with total ERK and got good signal and later on when i do stripping. After stripping the blot i prob the blot with phosphoERK antibody.
My total ERK gives very good signal, while there comes huge background in the phospho ERK probing.
Can anyone gives some suggestion.
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Huge background with HT22 cells phophoERK antibodyWestern blotting he
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