cdna library.clontech kit
Posted 12 December 2011 - 03:14 AM
i' constructed cdna library using clontech kit. but i didn't get any result when i used my stored competent cells which i made 4days, back stored in -80i.e colonies neither in control nor in negative (only competent cell). freshly prepared cells gave me results. it made electrocompetents. and during first transformation i we gave electric pluse of 1800voltsand during second transformation gv 1700 volt pluse.
now i'm confused is there any problem with my competent cells or in giving pluse or is there anyother problem. so please help me.
Posted 12 December 2011 - 11:57 AM
Posted 12 December 2011 - 10:43 PM