if anyone knew pl kind sent it to me
0
isolation of genomic DNA from chicken blood in conventional method
Started by drelamuruganvet, Dec 12 2011 12:43 AM
3 replies to this topic
#2
bob1
-
- Global Moderators
-
- 4,342 posts
Thelymitra pulchella
222
Excellent
Excellent
Posted 12 December 2011 - 11:54 AM
Look for a procedure called "buffy coat", then proceed from there.
#3
drelamuruganvet
-
- Members
-
- 4 posts
member
0
Neutral
Neutral
Posted 12 December 2011 - 07:20 PM
bob1, on 12 December 2011 - 11:54 AM, said:
Look for a procedure called "buffy coat", then proceed from there.
thank you, still since i am isolating from chicken blood i need to collect DNA from RBC also.. i am finding difficult in completely get rid off lysed RBC.
#4
AquaPlasmid
-
- Active Members
-
- 65 posts
Enthusiast
4
Neutral
Neutral
Posted 05 January 2012 - 09:20 PM
You could extract DNA directly from whole blood using one of our product AquaPreserve in three simple steps: 1) use AquaPreserve to lyse the blood cells and extract the DNA, 2) use ProSink to precipitate the proteins, and then 3) use isopropanol to precipitate the DNA. No buffy coat, no WBC fractionation, no RBC lysis, no hemoglobin digest, no phenol/chloroform, and no column. I am sure you will like it!
