Hi!
So, I have a purified protein, and I have to exchange the buffer (DTT interfering with downstream binding to the Ni column).
The problem is that I have very small volume (10 uL, 6 uM) of the protein?
Could anyone recommend a type of column to use?
Anyone has experience with low volumes?
Thanks a lot!!
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3 replies to this topic
#2
mdfenko
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Posted 12 December 2011 - 07:40 AM
you could use a sephadex g-25 spin column but you will significantly dilute your protein.
you can try drop dialysis.
you can try drop dialysis.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
hannna
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Posted 12 December 2011 - 03:38 PM
I thought that drop dialysis is used for nucleic acids. At least I use it for that only.
I will try sephadex g 25 column. But I'm still worried about the small volume.. :/
I will try sephadex g 25 column. But I'm still worried about the small volume.. :/
#4
hannna
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Posted 12 December 2011 - 03:53 PM
Actually I'm going to order this:
http://www.piercenet...04-BEC6E0635687
They say it works with very small volumes.
Does anyone have experaience with it?
http://www.piercenet...04-BEC6E0635687
They say it works with very small volumes.
Does anyone have experaience with it?
