Posted 11 December 2011 - 05:50 PM
So, I have a purified protein, and I have to exchange the buffer (DTT interfering with downstream binding to the Ni column).
The problem is that I have very small volume (10 uL, 6 uM) of the protein?
Could anyone recommend a type of column to use?
Anyone has experience with low volumes?
Thanks a lot!!
Posted 12 December 2011 - 07:40 AM
you can try drop dialysis.
genius does what it must
i do what i get paid to do
Posted 12 December 2011 - 03:38 PM
I will try sephadex g 25 column. But I'm still worried about the small volume.. :/