First of all tnx for your posts
The thing is that i have collegues that work on the same reader with the same kits and they do not have the same problem like me
and they do not run calibration every time
@ almost a doctor: every time is different, sometimes they are lower and sometimes the oposite. For example TSH control is out of range to the lower end, and fT4 is out of range but to the higher end.
This confuses me because when i run new calibration, the samples are fine, but in a couple of days on the same calibration curve, which seems correct, i run the tests and i get some odd values. After that i have to run new calibration and after the new calibration the results are ok.
@ PAO_ahac: At first also thought that the problem was in the kit, so i have changed it, this is my third kit and the problem is still here. I don't think that the problem is in the kit, maybe in the reader or i'm doing something wrong, but i'm not runing ELISA for the first time. (this is my first time runing TSH and fT4 on ELISA). Also, i have noticed something that i think it should not be hapening. When i put stop solution, the absorbance should be stable at least 15 minutes, after stop solution when i read for the first time i have one value for the absorbance, but when i read 5 minutes later i have higher values for the absorbance, the reaction is not stable? But i have changed three kits and i have the same results.
Anyway i'm desperate so much that i really need to find a solution for my problem, otherwise, i have bought the reader for nothing.
p.s. tnx a lot nad have i nice day!!!
Edited by Dzimi, 12 December 2011 - 09:26 AM.