Recently I did a nuclear extraction of my cells with the Cayman Chemical protocol here: http://www.caymanche...fs/10009277.pdf
It was getting very late so after resuspending the pellet in the Complete nuclear extraction buffer (the pellet was still intact) I froze the samples at -80 C.
Are these still salvageable - i.e. if I thawed them would I be able to just continue the steps as detailed in the protocol - or should I throw these away? Any tips on this would be much appreciated.
Thanks in advance!
Nuclear Extraction protocol
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