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Sonication - to completely lyse cells

protein extraction

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#1 @bhijit

@bhijit

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Posted 08 December 2011 - 11:32 PM

Hello there,


I am using this sonicator for first time - It has setting for pulse time & amplitude %.
I have expressed protein using BL21DE3 cells - I want to lyse the cells completely to seperate the soluble and pellet fraction - please suggest the amplitude and time for pulses
previously i used 50% and 5 pulses of 10 sec but the cells did not lyse completely - almost similar protein pattern was visible in both soluble and pellet fraction.

Thank you very much for the help :)





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