2 replies to this topic

[fpcc]

I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before

i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice

its this a problem for the transformation efficiency?

[bob1]

Probably, the conditions for the heat shock are timed based on the heating of a given volume for a specific time.  They are also dependent on the concentrations of particular salts in the transformation buffer, which is why you should not exceed 5% of the competent cell volume for addition of DNA.

[Biouday]

I dont understand w

fpcc, on 08 December 2011 - 03:45 PM, said:

I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before

i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice

its this a problem for the transformation efficiency?

I donot understand what you trying to ask. Theoretically what ever you did is completely wrong,But if you manage to get a clone you are lucky.