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bacterial transformation problem

transformation

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#1 fpcc

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Posted 08 December 2011 - 03:45 PM

I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before

i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice

its this a problem for the transformation efficiency?

#2 bob1

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Posted 08 December 2011 - 06:28 PM

Probably, the conditions for the heat shock are timed based on the heating of a given volume for a specific time. They are also dependent on the concentrations of particular salts in the transformation buffer, which is why you should not exceed 5% of the competent cell volume for addition of DNA.

#3 Biouday

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Posted 11 December 2011 - 03:16 PM

I dont understand w

I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before

i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice

its this a problem for the transformation efficiency?

I donot understand what you trying to ask. Theoretically what ever you did is completely wrong,But if you manage to get a clone you are lucky.





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