Posted 08 December 2011 - 08:28 AM
Using Thymidine @ 2.5mM to block Hela cells at G1/S boundary prior to lysis. Its my first time using the chemical and I didn't notice any morphological changes to the cells after 20 hrs. I've used HU before for this purpose and the cells definitely looked different after incubation. Am I doing something wrong or is this normal?
Posted 08 December 2011 - 06:36 PM
Posted 08 December 2011 - 08:00 PM
If anyone has experience with thymidine please let me know.
Edited by lysis_, 08 December 2011 - 08:01 PM.